abstract：:R-lactide, a pivotal monomer for the production of poly (D-lactic acid) (PDLA) or stereocomplex poly (lactic acid) (PLA) was synthesized from alkyl (R)-lactate through a lipase-catalyzed reaction without racemization. From among several types of lipase, only lipase B from Candida antarctica (Novozym 435; CAL-B) was ef...

journal_title：Journal of biotechnology

authors： Jeon BW,Lee J,Kim HS,Cho DH,Lee H,Chang R,Kim YH

更新日期：2013-10-20 00:00:00

abstract：:The aim of this study is to investigate the effects of an artificially controlled environment, particularly elevated total pressure, on net photosynthesis and respiration during plant growth. Pressure directly affects not only cells and organelles in leaves but also the diffusion coefficients and degrees of solubility...

journal_title：Journal of biotechnology

authors： Takeishi H,Hayashi J,Okazawa A,Harada K,Hirata K,Kobayashi A,Akamatsu F

更新日期：2013-10-20 00:00:00

abstract：:Turbulent flows in a single-stage and a two-stage impeller-stirred photobioreactor with a simple geometric configuration were analyzed using computational fluid dynamics. The trajectories of the microorganisms entrained in the flow field were traced by the particle tracking method. By projecting these trajectories ont...

journal_title：Journal of biotechnology

authors： Zhang T

更新日期：2013-10-10 00:00:00

abstract：:The complete genome sequence of Streptomyces fulvissimus (DSM 40593), consisting of a linear chromosome with a size of 7.9Mbp, is reported. Preliminary data indicates that the chromosome of S. fulvissimus contains 32 putative gene clusters involved in the biosynthesis of secondary metabolites, two of them showing very...

journal_title：Journal of biotechnology

authors： Myronovskyi M,Tokovenko B,Manderscheid N,Petzke L,Luzhetskyy A

更新日期：2013-10-10 00:00:00

abstract：:Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedure of direct oligon...

journal_title：Journal of biotechnology

authors： Jensen MA,Akhras MS,Fukushima M,Pourmand N,Davis RW

更新日期：2013-09-20 00:00:00

abstract：:Hydrophobic properties were conferred to a high-lignin-content Kraft pulp by a laccase-catalysed treatment in the presence of lauryl gallate (LG). The treatment resulted in a two-fold increase in contact angle and conferred water absorption resistance to the pulp. Kappa number was increased, indicating that some pheno...

journal_title：Journal of biotechnology

authors： Reynaud C,Tapin-Lingua S,Elegir G,Petit-Conil M,Baumberger S

更新日期：2013-09-10 00:00:00

abstract：:The objective of this study was to identify and isolate endogenous promoters in Chinese hamster ovary (CHO) cells using a promoter trap approach. A promoter-less vector harboring a green fluorescent protein (GFP)-hygromycin resistance gene cassette was designed and transfected into CHO cells. Putative promoters were i...

journal_title：Journal of biotechnology

authors： Chen J,Haverty J,Deng L,Li G,Qiu P,Liu Z,Shi S

更新日期：2013-09-10 00:00:00

abstract：:Early development datasets are typically unstructured, incomplete and truncated, yet they are readily available and contain relevant process information which is not extracted using classical data analysis techniques. In this paper, we illustrate the power of multivariate data analysis (MVDA) as a Process Analytical T...

journal_title：Journal of biotechnology

authors： Mercier SM,Diepenbroek B,Dalm MC,Wijffels RH,Streefland M

更新日期：2013-09-10 00:00:00

abstract：:Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to mainly contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. In addition the cell's essential functions are maintained under stress conditions like high salinity, heat or aridity stress. H...

journal_title：Journal of biotechnology

authors： Eilert E,Kranz A,Hollenberg CP,Piontek M,Suckow M

更新日期：2013-08-20 00:00:00

abstract：:Traditional strategies for production of thermo-induced recombinant protein in Escherichia coli consist of a two-phase culture, with an initial growth stage at low temperature (commonly 30°C) followed by a production stage where temperature is increased stepwise (commonly up to 42°C). A disadvantage of such strategies...

journal_title：Journal of biotechnology

authors： Caspeta L,Lara AR,Pérez NO,Flores N,Bolívar F,Ramírez OT

更新日期：2013-08-10 00:00:00

abstract：:A highly active and stable cross-linked enzyme aggregates (CLEAs) of epoxide hydrolases (EHs) from Mung bean, which plays a crucial role in synthesis of valuable enantiopure diols, were successfully prepared and characterized. Under the optimum preparation conditions, the activity recovery of CLEAs recorded 92%. The C...

journal_title：Journal of biotechnology

authors： Yu CY,Li XF,Lou WY,Zong MH

更新日期：2013-06-20 00:00:00

abstract：:Stem cell sensors have emerged as a promising technique to electrochemically monitor the functional status and viability of stem cells. However, efficient electrochemical analysis techniques are required for the development of effective electrochemical stem cell sensors. In the current study, we report a newly develop...

journal_title：Journal of biotechnology

authors： Yea CH,An JH,Kim J,Choi JW

更新日期：2013-06-20 00:00:00

abstract：:Interactions of cells with polymer-based biomaterials are influenced by properties of the substrate. Polymers, which are able to induce cell specific effects, gain increasing importance for biotechnology and regenerative therapies. A test system was developed, which allows studying primary human keratinocytes and fibr...

journal_title：Journal of biotechnology

authors： Trescher K,Roch T,Cui J,Kratz K,Lendlein A,Jung F

更新日期：2013-06-20 00:00:00

abstract：:Polyhydroxyalkanoates (PHA), a family of diverse bio-polyesters, are produced by many bacteria as an energy and carbon storage material. PHA synthesis regulatory protein PhaR was reported to attach on the surface of intracellular PHA granules for convenience of synthesis regulation. PhaR was found to have an amphiphil...

journal_title：Journal of biotechnology

authors： Ma HK,Liu MM,Li SY,Wu Q,Chen JC,Chen GQ

更新日期：2013-06-20 00:00:00

abstract：:The promoter sequences of the encoding genes for the three most abundant hexamerins of the Lepidoptera Trichoplusia ni were isolated and cloned into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-derived baculovirus expression vector. From the sequences analyzed, the DNA region driving the expressio...

journal_title：Journal of biotechnology

authors： López-Vidal J,Gómez-Sebastián S,Sánchez-Ramos I,Escribano JM

更新日期：2013-06-10 00:00:00

abstract：:Delivery of macromolecules into the cytosolic space of eukaryotic cells is a pressing challenge in biopharmaceutics. Macromolecules are often encapsulated into liposomes for protection and improved distribution, but the their size often induces endocytosis of the vehicle at the target site, leading to degradation of t...

journal_title：Journal of biotechnology

authors： Walls ZF,Goodell S,Andrews CD,Mathis J,Lee KD

更新日期：2013-04-15 00:00:00

abstract：:To improve enzymatic activity of Bacillus pumilus lipases, DNA shuffling was applied to two lipase genes from local B. pumilus isolates. Using a high-throughput activity assay, the mutant with highest activity was selected. This chimeric mutant (L3-3), carrying two crossover positions and three point mutations, has a ...

journal_title：Journal of biotechnology

authors： Akbulut N,Tuzlakoğlu Öztürk M,Pijning T,İşsever Öztürk S,Gümüşel F

更新日期：2013-03-10 00:00:00

abstract：:Saccharomyces cerevisiae can be engineered for xylose fermentation through introduction of wild type or mutant genes (XYL1/XYL1 (R276H), XYL2, and XYL3) coding for xylose metabolic enzymes from Scheffersomyces stipitis. The resulting engineered strains, however, often yielded undesirable phenotypes such as slow xylose...

journal_title：Journal of biotechnology

authors： Kim SR,Lee KS,Kong II,Lesmana A,Lee WH,Seo JH,Kweon DH,Jin YS

更新日期：2013-03-10 00:00:00

abstract：:We report molecular dynamic simulations showing that a DNA molecule constituted by five unique bases can be spontaneously inserted into single walled carbon nanotube (SWCNT) in normal conditions (P, T and water environment) depending on the tube radius value. The van der Waals and electrostatic interactions play a cen...

journal_title：Journal of biotechnology

authors： Clavier A,Kraszewski S,Ramseyer C,Picaud F

更新日期：2013-03-10 00:00:00

abstract：:The PRNP gene encodes a cellular protein named prion, whose misfolded form has been implicated in a number of neuropathic diseases in mammals such as the Bovine Spongiform Encephalopathy (BSE) in cattle. BSE has brought devastating impact on the world economy and human health. Recently, several groups have performed t...

journal_title：Journal of biotechnology

authors： Wang S,Zhang K,Ding F,Zhao R,Li S,Li R,Xu L,Song C,Dai Y,Li N

更新日期：2013-02-20 00:00:00

abstract：:In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver...

journal_title：Journal of biotechnology

authors： Ahmed V,Kumar J,Kumar M,Chauhan MB,Vij M,Ganguli M,Chauhan NS

更新日期：2013-02-20 00:00:00

abstract：:For proteome research, antibodies against a growing number of antigens must be generated and characterized. The high throughput generation of antibody fragments, using in vitro selection, requires bacterial expression of antibody fragments. This created a need to establish an expression method to improve the parallel ...

journal_title：Journal of biotechnology

authors： Miethe S,Meyer T,Wöhl-Bruhn S,Frenzel A,Schirrmann T,Dübel S,Hust M

更新日期：2013-01-20 00:00:00

abstract：:This study aimed to produce a cellulase blend and to evaluate its application in a simultaneous saccharification and fermentation (SSF) process for second generation ethanol production from sugar cane bagasse. The sugar cane bagasse was subjected to pretreatments (diluted acid and alkaline), as for disorganizing the l...

journal_title：Journal of biotechnology

authors： Maeda RN,Barcelos CA,Santa Anna LM,Pereira N Jr

更新日期：2013-01-10 00:00:00

abstract：:The increasing demand of monoclonal antibodies for therapeutic applications along with the high manufacturing cost have made it necessary to evaluate better process options and technologies for their purification. Affinity precipitation is an attractive alternative to traditional chromatographic methods by affording e...

journal_title：Journal of biotechnology

authors： Madan B,Chaudhary G,Cramer SM,Chen W

更新日期：2013-01-10 00:00:00

abstract：:Sulphite spent liquor (SSL) is a side product from acidic sulphite pulping of wood, which organic counterpart is composed mainly by lignosulphonates (LS) and sugars. The last are a prominent substrate for the bioprocessing although a previous purification step is necessary to eliminate microbial inhibitors. In this st...

journal_title：Journal of biotechnology

authors： Fernandes DL,Silva CM,Xavier AM,Evtuguin DV

更新日期：2012-12-31 00:00:00

abstract：:A comparative study was made of Mung bean epoxide hydrolases-catalyzed asymmetric hydrolysis of styrene oxide to (R)-1-phenyl-1,2-ethanediol in an n-hexane/buffer biphasic system containing various hydrophilic ionic liquids (ILs). Compared to the n-hexane/buffer biphasic system alone, addition of a small amount of hyd...

journal_title：Journal of biotechnology

authors： Chen WJ,Lou WY,Yu CY,Wu H,Zong MH,Smith TJ

更新日期：2012-12-31 00:00:00

abstract：:Multiple phage-peptide constructs, where the peptides mimic sperm epitopes that bind to zona pellucida (ZP) proteins, were generated via selection from a phage display library using a novel approach. Selections were designed to allow for identification of ZP-binding phage clones with potential species-specific propert...

journal_title：Journal of biotechnology

authors： Samoylova TI,Cochran AM,Samoylov AM,Schemera B,Breiteneicher AH,Ditchkoff SS,Petrenko VA,Cox NR

更新日期：2012-12-31 00:00:00

abstract：:The vitamin E family consists of 4 tocopherol and 4 tocotrienol compounds. During recent years, tocotrienols have gained increased interest due to their biological activities that are beyond the vitamin E activity. Here we report the engineering of plasmid-free Escherichia coli strains for an efficient synthesis of 2-...

journal_title：Journal of biotechnology

authors： Ghanegaonkar S,Conrad J,Beifuss U,Sprenger GA,Albermann C

更新日期：2012-12-15 00:00:00

abstract：:In this study, enzymatic transesterification of ethyl ferulate (EF) and monostearin for feruloylated lipids production was investigated. Enzyme screening and the effect of feruloyl acceptors on the transesterification were also studied. Effects of reaction variables (reaction temperatures, enzyme load, and reaction ti...

journal_title：Journal of biotechnology

authors： Sun S,Song F,Bi Y,Yang G,Liu W

更新日期：2012-12-15 00:00:00

abstract：:This work explores some of the possibilities to replace human cytomegalovirus (CMV) core and/or enhancer promoter control elements to create new expression vectors for use with fish. The work is relevant to fish vaccination, since DNA vaccines use eukaryotic expression plasmids controlled by the human cytomegalovirus ...

journal_title：Journal of biotechnology

authors： Martinez-Lopez A,Chinchilla B,Encinas P,Gomez-Casado E,Estepa A,Coll JM

更新日期：2012-12-15 00:00:00

abstract：:In this study, we used the homologous recombination protein RecA to locate a specific sequence on DNA. Single-stranded (ss) DNA (80-mer, 5'-biotinylated), complementary to the sequence of interest, was labeled with quantum dots (Qdots(®)) via biotin-avidin binding. The DNA was then mixed with RecA to form a fluorescen...

journal_title：Journal of biotechnology

authors： Kimura Y,Goto Y,Oana H,Washizu M

更新日期：2012-12-15 00:00:00

abstract：:The aim of this study was to investigate the effect of micronization on the compound content, crystalline structure and physicochemical properties of fiber from genetically modified (GM) flax. The GM flax was transformed with three bacterial (Ralstonia eutropha) genes coding for enzymes of polyhydroxybutyrate (PHB) sy...

journal_title：Journal of biotechnology

authors： Dymińska L,Szatkowski M,Wróbel-Kwiatkowska M,Zuk M,Kurzawa A,Syska W,Gągor A,Zawadzki M,Ptak M,Mączka M,Hanuza J,Szopa J

更新日期：2012-12-15 00:00:00

abstract：:Water shortage necessitated South Indian sago factory owners, extracting starch out of cassava tubers, to install biogas plants where a starch utilizing microbial community multiplies and reduces the biological oxygen demand (BOD) of the waste waters by presently about 30%. The purification efficiency of sago factory ...

journal_title：Journal of biotechnology

authors： Rajendran R,Soora M,Dananjeyan B,Ratering S,Krishnamurthy K,Benckiser G

更新日期：2012-12-15 00:00:00

abstract：:An increasing number of investors is looking at algae as a viable source of biofuels, beside cultivation for human/animal feeding or to extract high-value chemicals and pharmaceuticals. However, present biomass productivities are far below theoretical estimations implying that a large part of the available photosynthe...

journal_title：Journal of biotechnology

authors： Formighieri C,Franck F,Bassi R

更新日期：2012-11-30 00:00:00

abstract：:Microbiologically induced calcium carbonate precipitation (MICCP) is a naturally occurring biological process that has shown its potential in remediation of a wide range of structural damages including concrete cracks. In this study, genetically engineered microorganisms, capable of producing extracellular polymeric s...

journal_title：Journal of biotechnology

authors： Bergdale TE,Pinkelman RJ,Hughes SR,Zambelli B,Ciurli S,Bang SS

更新日期：2012-10-31 00:00:00

abstract：:Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membrane biofouling. Thi...

journal_title：Journal of biotechnology

authors： Siddiqui MF,Sakinah M,Singh L,Zularisam AW

更新日期：2012-10-31 00:00:00

abstract：:In this work it is describe for the first time, the use of an esterase with null activity (Tfu_0883 bacterial cutinase from Thermobifida fusca) on the removal of fat from the surface of a cotton substrate. Similar levels of fat removal were found for both null and wild-type proteins, despite that only wild type protei...

journal_title：Journal of biotechnology

authors： Silva C,Azoia NG,Martins M,Matamá T,Wu J,Cavaco-Paulo A

更新日期：2012-10-31 00:00:00

abstract：:Aiming at maximizing the production of transmembrane rabies virus glycoprotein (rRVGP), the influence of hypothermic temperature on a recombinant Drosophila melanogaster S2 cell culture in Sf-900II medium was investigated. Cell growth and rRVGP production were assessed at 4 culture temperatures in Schott flasks: 16, 2...

journal_title：Journal of biotechnology

authors： Rossi N,Silva BG,Astray R,Swiech K,Pereira CA,Suazo CA

更新日期：2012-10-31 00:00:00

abstract：:Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treat...

journal_title：Journal of biotechnology

authors： Lee JS,Kim YJ,Kim CL,Lee GM

更新日期：2012-09-15 00:00:00

abstract：:A microcarrier culture system was established for a large-scale production of functional human endothelial cells. It has been difficult to cultivate human endothelial cells in large quantities for the reasons that specific growth factor and extracellular matrix are required for the survival and proliferation of the ce...

journal_title：Journal of biotechnology

authors： Tashiro S,Tsumoto K,Sano E

更新日期：2012-08-31 00:00:00